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Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba
Auteur(s)
Charriere, Florian
Pavillon, Nicolas
Colomb, Tristan
Depeursinge, Christian
Heger, Thierry J
Marquet, Pierre
Rappaz, Benjamin
Date de parution
2006
In
Optics Express
Vol.
16
No
14
De la page
7005
A la page
7013
Résumé
This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of p, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20x magnification allows a resolution better than 3 mu m in all three dimensions, with accuracy better than 0.01 for the refractive index measurements. This technique is for the first time to our knowledge applied to living specimen ( testate amoeba, Protista). Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%. (c) 2006 Optical Society of America
Type de publication
Resource Types::text::journal::journal article
