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TRiFLe, a program for in silico terminal restriction fragment length polymorphism analysis with user-defined sequence sets
Auteur(s)
Date de parution
2008
In
Applied and environmental microbiology
Vol.
20
No
74
De la page
6452
A la page
6456
Résumé
We describe TRiFLe, a freely accessible computer program that generates theoretical terminal restriction fragments (T-RFs) from any user-supplied sequence set tailored to a particular group of organisms, sequences from clone libraries, or sequences from specific genes. The program allows a rapid identification of the most polymorphic enzymes, creates a collection of T-RFs for the data set, and can potentially identify specific T-RFs in T-RF length polymorphism (T-RFLP) patterns by comparing theoretical and experimental results. TRiFLE was used for analyzing T-RFLP data generated for the amoA and pmoA genes. The peaks identified in the T-RFLP patterns show an overlap of ammonia- and methane-oxidizing bacteria in the metalimnion of a subtropical lake.
Type de publication
Resource Types::text::journal::journal article
