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Aebi, Alexandre

Nom
Aebi, Alexandre
Affiliation principale
Site web
Fonction
MER
Email
alexandre.aebi@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/769
_
0000-0003-2309-1497

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  • Publication
    Métadonnées seulement
    Molecular gut content analysis for detection of intraguild predation by H. axyridis (Coleoptera: Coccinellidae) on non-coccinellid aphidophagous predators
    (2013-3-31)
    Ingels, Brecht
    ;
    Aebi, Alexandre 
    ;
    Hautier, Louis
    ;
    Van Leeuwen, Thomas
    ;
    De Clercq, Patrick
    Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.
  • Publication
    Métadonnées seulement
    Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids
    (2013)
    Ingels, Brecht
    ;
    Aebi, Alexandre 
    ;
    Hautier, Louis
    ;
    Van Leeuwen, T.
    ;
    De Clercq, Patrick
    Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.
  • Publication
    Accès libre
    Detecting arthropod intraguild predation in the field
    (2011)
    Aebi, Alexandre 
    ;
    Brown, Peter M. J.
    ;
    De Clercq, Patrick
    ;
    Hautier, Louis
    ;
    Howe, Andy
    ;
    Ingels, Brecht
    ;
    Ravn, Hans-Peter
    ;
    Sloggett, John J.
    ;
    Zindel, Renate
    ;
    Thomas, Alison
  • Publication
    Accès libre
    Detecting arthropod intraguild predation in the field
    Aebi, Alexandre 
    ;
    Brown, Peter M. J
    ;
    De Clercq, Patrick
    ;
    Hautier, Louis
    ;
    Howe, Andy
    ;
    Ingels, Brecht
    ;
    Ravn, Hans-Peter
    ;
    Sloggett, John J
    ;
    Zindel, Renate
    ;
    Thomas, Alison
    The process of biological control carries a distinct risk that an alien biological control agent (BCA) will become established as an invasive alien species with an associated threat to the local ecosystem biodiversity. It is imperative that a wide-ranging environmental risk assessment (ERA) is performed before the release of any BCA. This should include considering various potential but difficult to observe ecological interactions between the BCA and members of the native community, including disruption of intraguild relationships. Detection of intraguild predation (IGP) events involving predatory arthropods in the field can be done by analyzing their gut contents. Polymerase chain reaction (PCR) is a sensitive and specific tool to identify target prey DNA within a predator’s gut. This paper reviews the efficiency of a DNA based approach for detecting IGP in the field, compared with detection by the use of monoclonal antibodies or gas chromatography. Prey specificity, detection times after prey consumption, capacity for quantification, multiple prey targeting and the time and costs involved in developing and using the different methods are considered.