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Gern, Lise

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Gern, Lise
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lise.gern@unine.ch

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  • Publication
    Métadonnées seulement
  • Publication
    Métadonnées seulement
    Therapeutic passive vaccination against chronic Lyme disease in mice
    (1997)
    Zhong, Weimin
    ;
    Stehle, Thomas
    ;
    Museteanu, Crisan
    ;
    Siebers, Annette
    ;
    Gern, Lise 
    ;
    Kramer, Michael
    ;
    Wallich, Reinhard
    ;
    Simon, Markus M
    Passive and active immunization against outer surface protein A (OspA) has been successful in protecting laboratory animals against subsequent infection with Borrelia burgdorferi. Antibodies (Abs) to OspA convey full protection, but only when they are present at the time of infection, Abs inactivate spirochetes within the tick and block their transmission to mammals, but do not affect established infection because of the loss of OspA in the vertebrate host. Our initial finding that the presence of high serum titers of anti-OspC Abs (5 to 10 mu g/ml) correlates with spontaneous resolution of disease and infection in experimentally challenged immunocompetent mice suggested that therapeutic vaccination with OspC may be feasible. We now show that polyclonal and monospecific mouse immune sera to recombinant OspC, but not to OspA, of B. burgdorferi resolve chronic arthritis and carditis and clear disseminated spirochetes in experimentally infected C.B.-17 severe combined immunodeficient mice in a dose-dependent manner. This was verified by macroscopical and microscopical examination of affected tissues and recultivation of spirochetes from ear biopsies. Complete resolution of disease and infection was achieved, independent of whether OspC-specific immune sera (10 mu g OspC-specific Abs) were repeatedly given (4 x in 3- to 4-day intervals) before the onset (day 10 postinfection) or at the time of fully established arthritis and carditis (days 19 or 60 postinfection). The results indicate that in mice spirochetes constitutively express OspC and are readily susceptible to protective OspC-specific Abs throughout the infection. Thus, an OspC-based vaccine appears to be a candidate for therapy of Lyme disease.
  • Publication
    Métadonnées seulement
    Coinoculation of Borrelia spp. with tick salivary gland lysate enhances spirochete load in mice and is tick species-specific
    (2002)
    Zeidner, Nordin S
    ;
    Schneider, Bradley S
    ;
    Nuncio, Maria S
    ;
    Gern, Lise 
    ;
    Piesman, Joseph
    C3H/HeN mice were inoculated with 10(6) spirochetes, either Borrelia burgdorferi strain N40 or the Portuguese strain of B. lusitaniae, PotiB2. Mice receiving spirochetes comoculated with salivary gland lysate (SGL) demonstrated significantly higher spirochete loads in target organs as measured by quantitative real-time polymerase chain reaction. This effect was tick dependent. in that Ixodes ricinus SGL specifically enhanced B. lusitaniae load, whereas I. scapidaris SGL specifically increased B. burgdorferi N40 load, but did not significantly affect the dissemination of B. lusitaniae. Protein profile analysis indicated at least 5 major protein differences between I. scapiularis and I. ricinus SGL, which can possibly account for this specific tick-spirochete interaction.
  • Publication
    Métadonnées seulement
    PCR detection of granulocytic Ehrlichiae in Ixodes ricinus ticks and wild small mammals in western Switzerland
    (2000)
    Liz, Jorge S
    ;
    Anderes, Laurence
    ;
    Sumner, John W
    ;
    Massung, Robert F
    ;
    Gern, Lise 
    ;
    Rutti, Bernard
    ;
    Brossard, Michel 
    The presence of granulocytic ehrlichiae was demonstrated by PCR in Ixodes ricinus ticks and wild small mammals in Switzerland in two areas of endemicity for bovine ehrlichiosis. Six ticks (three females and three nymphs) (1.4%) of 417 I. ricinus ticks collected by flagging vegetation contained ehrlichial DNA. A total of 201 small mammals from five species, wood mouse (Apodemus sylvaticus), yellow-necked mouse (Apodemus flavicollis), earth vole (Pitymys subterraneus), bank vole (Clethrionomys glareolus), and common shrew (Sorex araneus), were trapped. The analysis of I. ricinus mammals collected on 116 small mammals showed that nine C. glareolus voles and two A. sylvaticus mice hosted infected tick larvae. In these rodents, granulocytic ehrlichia infection was also detected in blood, spleen, liver, and ear samples. Further examinations of 190 small mammals without ticks or with noninfected ticks showed the presence of ehrlichial DNA in spleen and other tissues from six additional C. glareolus, three A. flavicollis, and one S. araneus mammals. This study suggests that A. sylvaticus, A. flavicollis, S. araneus, and particularly C. glareolus are likely to be natural reservoirs for granulocytic ehrlichiae. Partial 16S rRNA gene sequences of granulocytic ehrlichiae from ticks and rodents showed a high degree of homology (99 to 100%) with granulocytic ehrlichiae isolated from humans. In contrast, groESL heat shock operon sequence analysis showed a strong divergence (approximately 5%) between the sequences in samples derived from rodents and those derived from samples from questing ticks or from other published ehrlichia sequences. Dual infections with granulocytic ehrlichia and Borrelia burgdorferi were found in ticks and small mammals.
  • Publication
    Accès libre
    Lyme disease
    Gern, Lise 
    ;
    Falco, R.C
    La borréliose de Lyme qui est, dans l'hémisphère nord, la plus répandue des maladies à transmission vectorielle, est due à des bactéries appartenant au genre Borrelia burgdorferi. Il s'agit d'une maladie plurisystémique qui affecte essentiellement la peau, le système nerveux le cœur et les articulations. En Europe, le vecteur de la maladie est la tique Ixodes ricinus; aux États -Unis d'Amérique les deux vecteurs principaux sont la tique I. scapularis dans les régions du nord-est et du centre-ouest et I. pacificus sur la Côte Ouest. Plusieurs espèces de petits et moyens mammifères ou d'oiseaux terrestres servent de réservoirs à la bactérie dans les zones endémiques. Le pronostic est excellent, surtout lorsque la maladie de Lyme est diagnostiquée en phase initiale et traitée immédiatement. La prophylaxie repose sur deux méthodes: l'immunisation préventive, d'une part, et la mise en œuvre de mesures permettant d'éviter les piqûres de tiques et de lutter contre ces vecteurs, d'autre part., Lyme borreliosis, the most common vector-bome disease in the northern hemisphere, is caused by bacteria belonging to the Borrelia burgdorferi complex. The disease is multisystemic, affecting mainly the skin, nervous system, heart and joints. In Europe, the vector of the disease is the tick Ixodes ricinus, whereas in the United States of America, two primary tick vectors exist, namely: I. scapularis in the north-eastern and mid-western regions and I. pacificus on the west coast. Several species of small and medium-sized mammals and ground-feeding birds serve as reservoirs for the bacteria in endemic areas. The prognosis for patients with Lyme borreliosis is excellent, particularly when diagnosed and treated early in the course of infection. Prevention of Lyme borreliosis can be achieved using two approaches, either prevention of infection by immunisation, or prevention of tick bites through avoidance, personal protection and tick control., De todas las enfermedades vehiculadas por vectores, la borreliosis de Lyme, causada por bacterias pertenecientes al complejo Borrelia burgdorferi, es la más común en el hemisferio norte. Esta enfermedad de carácter multisistémico afecta principalmente la piel, el sistema nervioso, el corazón y las articulaciones. En Europa, el vector de la enfermedad es la garrapata Ixodes ricinus. En los Estados Unidos de América, por su parte, existen dos garrapatas que ejercen de vectores primarios: I.scapularis en las regiones del noreste y el medio oeste, e I.pacificus en la costa oeste. En las áreas endémicas varias especies de mamíferos pequeños y medianos y de aves de alimentación terrestre sirven de reservorio de las bacterias. Los pacientes afectados de borreliosis de Lyme presentan un pronóstico excelente, sobre todo cuando se diagnostica y trata la infección en sus fases iniciales. Para prevenir esta enfermedad caben dos procedimientos distintos: prevenir la infección con medidas de inmunización; o impedir la mordedura de la garrapata previniendo esta posibilidad, protegiéndose del contacto y luchando contra sus poblaciones.
  • Publication
    Métadonnées seulement
    Tick-borne pathogens in the blood of wild and domestic ungulates in South Africa: interplay of game and livestock
    (2014)
    Berggoetz, M
    ;
    Schmid, M
    ;
    Ston, D
    ;
    Wyss, V
    ;
    Chevillon, Christophe
    ;
    Pretorius, A-M
    ;
    Gern, Lise 
  • Publication
    Métadonnées seulement
  • Publication
    Métadonnées seulement
    Longterm survey (7 years) in a population at risk for Lyme borreliosis: What happens to the seropositive individuals?
    (1998)
    Fahrer, Heinz
    ;
    Sauvain, Marie-Josephe
    ;
    Zhioua, Elyes
    ;
    Van Hoecke, C
    ;
    Gern, Lise