Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis
Amaya Vilches Barro, Dorothee Stöckle, Martha Thellmann, Paola Ruiz-Duarte, Lotte Bald, Marion Louveaux, Patrick Von Born, Philipp Denninger, Tatsuaki Goh, Hidehiro Fukaki, Joop Vermeer & Alexis Maizel
| Résumé |
How plant cells re-establish differential growth to initiate organs
is poorly understood. Morphogenesis of lateral roots relies on the
asymmetric cell division of initially symmetric founder cells. This
division is preceded by the tightly controlled asymmetric radial
expansion of these cells. The cellular mechanisms that license and
ensure the coordination of these events are unknown. Here, we
quantitatively analyze microtubule and F-actin dynamics during
lateral root initiation. Using mutants and pharmacological and
tissue-specific genetic perturbations, we show that dynamic
reorganization of both microtubule and F-actin networks is
necessary for the asymmetric expansion of the founder cells. This
cytoskeleton remodeling intertwines with auxin signaling in the
pericycle and endodermis in order for founder cells to acquire a
basic polarity required for initiating lateral root development.
Our results reveal the conservation of cell remodeling and
polarization strategies between the Arabidopsis zygote and lateral
root founder cells. We propose that coordinated, auxin-driven
reorganization of the cytoskeleton licenses asymmetric cell growth
and divisions during embryonic and post-embryonic organogenesis. |
| Mots-clés |
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| Citation | Vilches Barro, A., Stöckle, D., Thellmann, M., Ruiz-Duarte, P., Bald, L., Louveaux, M., von Born, P., Denninger, P., Goh, T., Fukaki, H., Vermeer, J., & Maizel, A. (2019). Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis. Current Biology, 29, 2443-2454. |
| Type | Article de périodique (Anglais) |
| Date de publication | 18-7-2019 |
| Nom du périodique | Current Biology |
| Volume | 29 |
| Pages | 2443-2454 |
| URL | https://www.sciencedirect.com/science/article/pii/S096098... |
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