Metabolomics reveals herbivore-induced metabolites of resistance and susceptibility in maize leaves and roots
Guillaume Marti, Matthias Erb, J. Boccard, Gaëtan Glauser, G. R. Doyen, Neil Villard, Christelle Aurélie Maud Robert, Ted Turlings, S. Rudaz & Jean-Luc Wolfender
| Résumé |
Plants respond to herbivory by reprogramming their metabolism. Most
research in this context has focused on locally induced compounds
that function as toxins or feeding deterrents. We developed an
ultra-high-pressure liquid chromatography time-of-flight mass
spectrometry (UHPLC-TOF-MS)-based metabolomics approach to evaluate
local and systemic herbivore-induced changes in maize leaves, sap,
roots and root exudates without any prior assumptions about their
function. Thirty-two differentially regulated compounds were
identified from Spodoptera littoralis-infested maize seedlings and
isolated for structure assignment by microflow nuclear magnetic
resonance (CapNMR). Nine compounds were quantified by a high
throughput direct nano-infusion tandem mass spectrometry/mass
spectrometry (MS/MS) method. Leaf infestation led to a marked local
increase of 1,3-benzoxazin-4-ones, phospholipids,
N-hydroxycinnamoyltyramines, azealic acid and tryptophan. Only few
changes were found in the root metabolome, but
1,3-benzoxazin-4-ones increased in the vascular sap and root
exudates. The role of N-hydroxycinnamoyltyramines in plantherbivore
interactions is unknown, and we therefore tested the effect of the
dominating p-coumaroyltyramine on S. littoralis. Unexpectedly,
p-coumaroyltyramine was metabolized by the larvae and increased
larval growth, possibly by providing additional nitrogen to the
insect. Taken together, this study illustrates that herbivore
attack leads to the induction of metabolites that can have
contrasting effects on herbivore resistance in the leaves and
roots. |
| Mots-clés |
zea mays, benzoxazinones, induced defence, induced resistance, metabolomics, p-coumaroyltyramine, root exudates, spodoptera littoralis, systemic signaling, uhplc-tof-ms, plant secondary metabolism, flight mass-spectrometry, below-ground herbivory, hdmboa-glc, natural benzoxazinones, arabidopsis-thaliana, bacterial pathogen, insect herbivores, innate immunity, jasmonic acid |
| Citation | Marti, G., Erb, M., Boccard, J., Glauser, G., Doyen, G. R., Villard, N., Robert, C. A. M., Turlings, T., Rudaz, S., & Wolfender, J. L. (2013). Metabolomics reveals herbivore-induced metabolites of resistance and susceptibility in maize leaves and roots. Plant, Cell and Environment, 36(3), 621-639. |
| Type | Article de périodique (Anglais) |
| Date de publication | 2013 |
| Nom du périodique | Plant, Cell and Environment |
| Volume | 36 |
| Numéro | 3 |
| Pages | 621-639 |