Geometric cues forecast the switch from two- to three-dimensional growth in Physcomitrella patens
Han Tang, Kilian Duijts, Magdalena Bezanilla, Ben Scheres, Joop Vermeer & Viola Willemsen
| Résumé |
During land colonization, plants acquired a range of body plan
adaptations, of which the innovation of three-dimensional (3D)
tissues increased organismal complexity and reproductivity. In the
moss, Physcomitrella patens, a 3D leafy gametophore originates from
filamentous cells that grow in a two-dimensional (2D) plane through
a series of asymmetric cell divisions. Asymmetric cell divisions
that coincide with different cell division planes and growth
directions enable the developmental switch from 2D to 3D, but
insights into the underlying mechanisms coordinating this switch
are still incomplete. Using 2D and 3D imaging and image
segmentation, we characterized two geometric cues, the width of the
initial cell and the angle of the transition division plane, which
sufficiently distinguished a gametophore initial cell from a branch
initial cell. These identified cues were further confirmed in
gametophore formation mutants. The identification of a fluorescent
marker allowed us to successfully predict the gametophore initial
cell with > 90% accuracy before morphological changes, supporting
our hypothesis that, before the transition division, parental cells
of the gametophore initials possess different properties from those
of the branch initials. Our results suggest that the cell fate
decision of the initial cell is determined in the parental cell,
before the transition division. |
| Mots-clés |
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| Citation | Tang, H., Duijts, K., Bezanilla, M., Scheres, B., Vermeer, J., & Willemsen, V. (2020). Geometric cues forecast the switch from two- to three-dimensional growth in Physcomitrella patens. New Phytologist, 225(5), 1945-1955. |
| Type | Article de périodique (Anglais) |
| Date de publication | 3-12-2020 |
| Nom du périodique | New Phytologist |
| Volume | 225 |
| Numéro | 5 |
| Pages | 1945-1955 |
| URL | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.16276 |
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